Swate template MIAPE - Chromatography
MIAPE, Proteomics, Chromatography
v1.0.1 released on 01/01/0001
143 Downloads
Template Content
Building Blocks + example values
| Input [Sample Name] | Characteristic [sample description] | Term Source REF (DPBO:0000175) | Term Accession Number (DPBO:0000175) | Parameter [sample preparation description] | Term Source REF (AFR:0002202) | Term Accession Number (AFR:0002202) | Parameter [injection (chromatography)] | Term Source REF (AFP:0001611) | Term Accession Number (AFP:0001611) | Component [chromatography column model] | Term Source REF (DPBO:0000048) | Term Accession Number (DPBO:0000048) | Parameter [chromatography column separation mode] | Term Source REF (AFR:0003034) | Term Accession Number (AFR:0003034) | Parameter [chromatography column length] | Unit | Term Source REF (AFR:0002029) | Term Accession Number (AFR:0002029) | Parameter [chromatography column inner diameter] | Unit | Term Source REF (CHMO:0002753) | Term Accession Number (CHMO:0002753) | Parameter [stationary phase] | Term Source REF (CHMO:0000986) | Term Accession Number (CHMO:0000986) | Parameter [chromatography column accessories] | Term Source REF (DPBO:0000138) | Term Accession Number (DPBO:0000138) | Component [chromatography instrument model] | Term Source REF (DPBO:0000046) | Term Accession Number (DPBO:0000046) | Parameter [mobile phase] | Term Source REF (CHMO:0000995) | Term Accession Number (CHMO:0000995) | Parameter [mobile phase constituents] | Term Source REF (DPBO:0000139) | Term Accession Number (DPBO:0000139) | Parameter [column run time] | Unit | Term Source REF (DPBO:0000140) | Term Accession Number (DPBO:0000140) | Parameter [chromatography gradient] | Term Source REF (DPBO:0000081) | Term Accession Number (DPBO:0000081) | Parameter [flow rate] | Term Source REF (AFR:0001881) | Term Accession Number (AFR:0001881) | Parameter [chromatography temperature program] | Term Source REF (CHMO:0002754) | Term Accession Number (CHMO:0002754) | Parameter [pre and post chromatography run processes] | Term Source REF (DPBO:0000142) | Term Accession Number (DPBO:0000142) | Parameter [chromatography detector] | Term Source REF (OBI:0000481) | Term Accession Number (OBI:0000481) | Component [detector model] | Term Source REF (DPBO:0000141) | Term Accession Number (DPBO:0000141) | Parameter [detector setting] | Term Source REF (AFR:0001247) | Term Accession Number (AFR:0001247) | Parameter [retention time acquisition range] | Term Source REF (MS:4000070) | Term Accession Number (MS:4000070) | Output [Data] | Data Format | Data Selector Format |
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| 100 mg frozen leaf tissue | extraction with chloroform, trimethylsilyl derivatization | 2 µl injected, 10 injections | Zebron ZB-AAA GC | reversed phase column | OBI | https://bioregistry.io/OBI:0000510 | 10 | meter | UO | https://bioregistry.io/UO:0000008 | 0.25 | millimeter | UO | https://bioregistry.io/UO:0000016 | C18 | SH-IP Guard Column | Shimadzu GCMS-QP2010 Ultra | mobile phase A and B | A: Water + 0.1% formic acid, B: Acetonitrile + 0.1% formic acid | 90 | minute | UO | https://bioregistry.io/UO:0000031 | 5–35% B over 90 minutes | 300 nL/min | start at 25 °C, increase 2°C/min, final 50°C | washing with methanol for 10 min at 0.5 mL/min | UV detector | Agilent 1260 Infinity II VWD | ESI+ | 5-50 min |