Swate template minSCe - Single cell isolation
single-cell RNA sequencing assay, RNA sequencing assay, single cell isolation, minSCe, Transcriptomics
v1.0.0 released on 05/15/2025
120 Downloads
Template Content
Building Blocks + example values
| Input [Sample Name] | Protocol Type | Term Source REF (DPBO:1000161) | Term Accession Number (DPBO:1000161) | Protocol REF | Protocol Description | Parameter [sample dissociation] | Term Source REF (EFO:0009091) | Term Accession Number (EFO:0009091) | Parameter [dissociation enzyme] | Term Source REF (SCEO:0000055) | Term Accession Number (SCEO:0000055) | Parameter [sample enrichment] | Term Source REF (EFO:0009106) | Term Accession Number (EFO:0009106) | Parameter [enrichment markers] | Term Source REF (SCEO:0000013) | Term Accession Number (SCEO:0000013) | Parameter [isolation of cell population] | Term Source REF (OBI:0000512) | Term Accession Number (OBI:0000512) | Parameter [single cell entity] | Term Source REF (SCEO:0000043) | Term Accession Number (SCEO:0000043) | Parameter [single cell quality metric] | Term Source REF (SCEO:0000044) | Term Accession Number (SCEO:0000044) | Parameter [Number of Cells] | Unit | Term Source REF (NCIT:C202259) | Term Accession Number (NCIT:C202259) | Parameter [single cell identifier] | Term Source REF (EFO:0010197) | Term Accession Number (EFO:0010197) | Output [Sample Name] |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| protoplasting procotol | SCEO | http://purl.obolibrary.org/obo/SCEO_0000047 | enzymatic dissociation | EFO | https://bioregistry.io/EFO:0009128 | macerozyme R-10 | SCEO | http://purl.obolibrary.org/obo/SCEO_0000056 | fluorescence-activated cell sorting | EFO | https://bioregistry.io/EFO:0009108 | CD45+ | Droplet-based Single-cell Isolation | NCIT | https://bioregistry.io/NCIT:C202598 | plant protoplast | PO | https://www.ebi.ac.uk/ols4/ontologies/po/classes/http%253A%252F%252Fpurl.obolibrary.org%252Fobo%252FPO_0000006 | OK | SCEO | http://purl.obolibrary.org/obo/SCEO_0000075 | 6284 | count unit | UO | https://bioregistry.io/UO:0000189 | extr_cell_1 |
Release Notes
Currently Work in Progress 🚧Versions
| Version | Name | Release on |
| 1.0.0 | minSCe - Single cell isolation | 05/15/2025 |