Swate template minSCe - Single cell library construction
single-cell RNA sequencing assay, RNA sequencing assay, library preparation, minSCe, Transcriptomics
v1.0.1 released on 05/22/2025
29 Downloads
Template Content
Building Blocks + example values
| Input [Sample Name] | Protocol Type | Term Source REF (DPBO:1000161) | Term Accession Number (DPBO:1000161) | Protocol REF | Protocol Description | Parameter [single cell library construction] | Term Source REF (EFO:0010183) | Term Accession Number (EFO:0010183) | Parameter [biological macromolecule] | Term Source REF (EFO:0004446) | Term Accession Number (EFO:0004446) | Parameter [primer] | Term Source REF (EFO:0010192) | Term Accession Number (EFO:0010192) | Parameter [nucleic acid amplification] | Term Source REF (EFO:0009107) | Term Accession Number (EFO:0009107) | Parameter [amplification cycles] | Unit | Term Source REF (SCEO:0000019) | Term Accession Number (SCEO:0000019) | Parameter [end bias] | Term Source REF (EFO:0010187) | Term Accession Number (EFO:0010187) | Parameter [Library Strand Sequenced] | Term Source REF (NCIT:C177563) | Term Accession Number (NCIT:C177563) | Parameter [spike in] | Term Source REF (EFO:0010193) | Term Accession Number (EFO:0010193) | Parameter [spike in dilution] | Unit | Term Source REF (EFO:0010217) | Term Accession Number (EFO:0010217) | Output [Sample Name] |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| nucleic acid library construction protocol | EFO | https://bioregistry.io/EFO:0004184 | Drop-seq | EFO | https://bioregistry.io/EFO:0008722 | polyA RNA | SCEO | http://purl.obolibrary.org/obo/SCEO_0000051 | oligo-dT | EFO | https://bioregistry.io/EFO:0010215 | polymerase chain reaction | OBI | https://bioregistry.io/OBI:0000415 | 15 | count unit | UO | https://bioregistry.io/UO:0000189 | 3 prime end bias | EFO | https://bioregistry.io/EFO:0010189 | first strand | SCEO | http://purl.obolibrary.org/obo/SCEO_0000081 | ERCC mix 1 | SCEO | http://purl.obolibrary.org/obo/SCEO_0000029 | 1:40000 | ratio | UO | https://bioregistry.io/UO:0010006 |