Swate template MIAPE - Gel electrophoresis
Gel Electrophoresis, Proteomics, MIAPE
v1.0.0 released on 01/01/0001
145 Downloads
Template Content
Building Blocks + example values
| Input [Sample Name] | Parameter [gel electrophoresis dimension type] | Term Source REF (DPBO:0000143) | Term Accession Number (DPBO:0000143) | Component [loading buffer] | Term Source REF (DPBO:0000144) | Term Accession Number (DPBO:0000144) | Parameter [gel electrophoresis dimension] | Term Source REF (DPBO:0000174) | Term Accession Number (DPBO:0000174) | Parameter [gel electrophoresis separation method] | Term Source REF (DPBO:0000145) | Term Accession Number (DPBO:0000145) | Parameter [gel matrix type] | Term Source REF (DPBO:0000146) | Term Accession Number (DPBO:0000146) | Parameter [gel manufacture] | Term Source REF (DPBO:0000147) | Term Accession Number (DPBO:0000147) | Parameter [physical dimensions of the gel] | Term Source REF (DPBO:0000148) | Term Accession Number (DPBO:0000148) | Parameter [physicochemical property range and distribution of the gel] | Term Source REF (DPBO:0000149) | Term Accession Number (DPBO:0000149) | Parameter [acrylamide concentration] | Term Source REF (DPBO:0000150) | Term Accession Number (DPBO:0000150) | Parameter [acrylamide crosslinker ratio] | Term Source REF (DPBO:0000151) | Term Accession Number (DPBO:0000151) | Parameter [additional gel ingredients] | Term Source REF (DPBO:0000152) | Term Accession Number (DPBO:0000152) | Parameter [gel lane number] | Term Source REF (DPBO:0000153) | Term Accession Number (DPBO:0000153) | Parameter [gel electrophoresis sample application] | Term Source REF (DPBO:0000154) | Term Accession Number (DPBO:0000154) | Parameter [running buffer] | Term Source REF (CHMO:0002501) | Term Accession Number (CHMO:0002501) | Parameter [electrophoresis conditions] | Term Source REF (DPBO:0000155) | Term Accession Number (DPBO:0000155) | Parameter [interdimension process] | Term Source REF (DPBO:0000156) | Term Accession Number (DPBO:0000156) | Output [Data] | Data Format | Data Selector Format |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| one-dimensional | Laemmli buffer | first | sodium dodecyl sulfate polyacrylamide gel electrophoresis | chmo | http://purl.obolibrary.org/obo/CHMO_0001027 | slab gel with gradient | gel_recipe.txt | 10 x 10 x 0.5 cm | linear pH 4-7, logarithmic apparent molecular mass 200-10 kDa | 1-2 % | 29:1 | 12 | well loading, 15 µl per well | Tris-Glycine-SDS buffer | 120 V, 50 min |
Release Notes
Currently Work in Progress 🚧Versions
| Version | Name | Release on |
| 1.0.0 | MIAPE - Gel electrophoresis | 01/01/0001 |