41f67274-1097-4144-9422-40b72febe911

Swate template Golden Gate cloning

v1.0.0 released on 01/01/0001

by Viktoria Petrova

145 Downloads

Template Content

Building Blocks + example values

Input [Source Name]	Protocol REF	Component [Ingredient]	Term Source REF (NCIT:C51981)	Term Accession Number (NCIT:C51981)	Parameter [Concentration]	Term Source REF (NCIT:C41185)	Term Accession Number (NCIT:C41185)	Parameter [Concentration]	Unit	Term Source REF (NCIT:C41185)	Term Accession Number (NCIT:C41185)	Parameter [Volume]	Unit	Term Source REF (NCIT:C25335)	Term Accession Number (NCIT:C25335)	Parameter [Final volume]	Unit	Term Source REF ()	Term Accession Number ()	Output [Sample Name]
		T4 Ligase Buffer			10x							1.5	Microliter	NCIT	https://bioregistry.io/NCIT:C48153	15	Microliter	NCIT	https://bioregistry.io/NCIT:C48153	cloning_master_mix
		T4 Ligase										0.75	Microliter	NCIT	https://bioregistry.io/NCIT:C48153	15	Microliter	NCIT	https://bioregistry.io/NCIT:C48153	cloning_master_mix
		BsaI-HFv2										0.5	Microliter	NCIT	https://bioregistry.io/NCIT:C48153	15	Microliter	NCIT	https://bioregistry.io/NCIT:C48153	cloning_master_mix
		destination plasmid						20	femtomolar	UO	https://bioregistry.io/UO:0000073	to be calculated	Microliter	NCIT	https://bioregistry.io/NCIT:C48153	15	Microliter	NCIT	https://bioregistry.io/NCIT:C48153	cloning_master_mix
		insert(s)						20	femtomolar	UO	https://bioregistry.io/UO:0000073	to be calculated	Microliter	NCIT	https://bioregistry.io/NCIT:C48153	15	Microliter	NCIT	https://bioregistry.io/NCIT:C48153	cloning_master_mix
		ddH2O										to be calculated	Microliter	NCIT	https://bioregistry.io/NCIT:C48153	15	Microliter	NCIT	https://bioregistry.io/NCIT:C48153	cloning_master_mix

Release Notes

Currently Work in Progress 🚧

Versions

Version	Name	Release on
1.0.0	Golden Gate cloning	01/01/0001