Swate template RNA extraction
v1.2.0 released on 01/01/0001
by Hajira Jabeen, Dominik Brilhaus
145 Downloads
Template Content
Building Blocks + example values
| Input [Source Name] | Protocol Type | Term Source REF (DPBO:1000161) | Term Accession Number (DPBO:1000161) | Protocol REF | Parameter [biosource amount] | Unit | Term Source REF (DPBO:0000013) | Term Accession Number (DPBO:0000013) | Parameter [extraction method] | Term Source REF (DPBO:0000054) | Term Accession Number (DPBO:0000054) | Parameter [extraction buffer] | Term Source REF (DPBO:0000050) | Term Accession Number (DPBO:0000050) | Parameter [extraction buffer volume] | Unit | Term Source REF (DPBO:0000051) | Term Accession Number (DPBO:0000051) | Parameter [RNA quality check] | Term Source REF (DPBO:0000062) | Term Accession Number (DPBO:0000062) | Output [Sample Name] |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| RNA extraction protocol | DPBO | http://purl.org/nfdi4plants/ontology/dpbo/DPBO_1000173 | rna-extraction.txt | QIAGEN RNEasy | user-specific | QIAGEN RNEasy Buffer 2 | user-specific | 200 | microliter | UO | https://bioregistry.io/UO:0000101 | RIN: 8.1 | user-specific | |||||||||
| PCI method | user-specific | phenol:chloroform:isopropanol | user-specific | microliter | UO | https://bioregistry.io/UO:0000101 | RNA quality checked by PAGE | user-specific | ||||||||||||||
| microliter | UO | https://bioregistry.io/UO:0000101 | ||||||||||||||||||||
| microliter | UO | https://bioregistry.io/UO:0000101 |