Swate template Imaging assay
v1.0.3 released on 01/01/0001
145 Downloads
Template Content
Building Blocks + example values
| Input [Source Name] | Parameter [target compartment] | Term Source REF (DPBO:1000121) | Term Accession Number (DPBO:1000121) | Parameter [microscope] | Term Source REF (OBI:0400169) | Term Accession Number (OBI:0400169) | Parameter [digital camera] | Term Source REF (OBI:0001048) | Term Accession Number (OBI:0001048) | Parameter [Objective] | Term Source REF (OME:Objective) | Term Accession Number (OME:Objective) | Parameter [magnification] | Term Source REF (DPBO:1000140) | Term Accession Number (DPBO:1000140) | Parameter [microscope filter] | Term Source REF (DPBO:1000125) | Term Accession Number (DPBO:1000125) | Parameter [Imaging Software Name] | Term Source REF (NCIT:C175898) | Term Accession Number (NCIT:C175898) | Parameter [imaging software settings] | Term Source REF (DPBO:1000124) | Term Accession Number (DPBO:1000124) | Parameter [imaging software version] | Term Source REF (DPBO:1000123) | Term Accession Number (DPBO:1000123) | Parameter [Assay Name] | Term Source REF (NCIT:C178857) | Term Accession Number (NCIT:C178857) | Parameter [dataset name] | Term Source REF (DPBO:1000126) | Term Accession Number (DPBO:1000126) | Parameter [image dimensionality] | Term Source REF (DPBO:1000141) | Term Accession Number (DPBO:1000141) | Parameter [raw data file format] | Term Source REF (DPBO:0000021) | Term Accession Number (DPBO:0000021) | Parameter [image file path] | Term Source REF (DPBO:1000120) | Term Accession Number (DPBO:1000120) | Output [Data] |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| organelles | OperaLX spinning-disk confocal microscope (Perkin Elmer) | user-specific | CoolSNAP HQ 1.4; Photometrics | user-specific | Leica HCX PL APO 100x, NA 1.47 Oil CORR TIRF PIFOC objective | additional 1.6x magnification lens | DIC | MetaXpress software | Image Dimension (XYZCT): 1376 x 1040 x 16 x 2 x 1 | OME-TIFF | |||||||||||||||||||||||||||||||||
| single cells | epifluorescence microscope (IX-81; Olympus-Europe, Hamburg, Germany) | user-specific | Cascade II 512 _ 512, Photometrics | user-specific | FITC | TIFF | |||||||||||||||||||||||||||||||||||||
| colony |